Renata F Erberelli, Renato M Salgado, Francisco J Lopes, Carlos Gilberto Almodin, Paula M Almodin, Philip Wolff
JBRA Assist. Reprod. 2012; 16 (4):247-249
Received July 17, 2022
Accepted December 01, 2022
Abstract
This paper reports on the birth of a healthy child after the transfer of pronuclear stage embryos cryopreserved through slow-freezing method, stored for nine years, and warmed through a standard devitrification protocol. Surviving embryos were transferred to a 45-year-old patient, resulting in clinical pregnancy and the birth of a live child. This case report demonstrated that long-term-stored pronuclear stage embryos cryopreserved through the slow-freezing method may successfully present cleavage, implantation and developmental viability after being warmed using a standard devitrification protocol.