Table 1. Sequence and specifications of primers. | ||
Primer | Sequence | PCR product length (bp) |
---|---|---|
ob-Rb | 5' CTGTGTAGTGTGAGGAGG 3' 5’ AAGGGAGGCACCGATGG 3’ |
109 |
ob-Ra | 5' CTCTTGTGTCCTGCTGC 3' 5’ GACTGTTGGGAGGTTGG 3’ |
107 |
NPY | 5' CCCAGAGCAGAGCACC 3' 5’ AGCAGGGATAGAGCGAG 3’ |
104 |
Bdnf | 5' CCCTTCTACACTTTACCTC 3' 5’ TTCACCCTTTCCACTCCT 3’ |
202 |
Kiss-1 | 5' TGCTGCTTCTCCTCTGTG 3' 5’ ACGAGTTCCTGGGGTCC 3’ |
106 |
β-actin | 5' CCATCTATGAGGGTTACGC 3' 5’ TGTAGCCACGCTCGGTC 3’ |
104 |